User:Javier Vinals Camallonga/Notebook/Javier Vinals notebook/2014/03/25

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Objective

Today we'll be adding three different concentrations of 30,60, and 90 mM to the 30:1 lysozyme AuNP solutions at three temperatures (4, 22, 37 degrees). Then we will synthesize an additional tube of 30:1 lysozyme AuNP to use as a standard future testing.

Procedure

Preparing Samples for Addition of Variables

  • Pipette 5 mL aliquots into a Falcon tube from the 15 mL samples.


Dilution of Variables

  • The following table shows how we diluted the stock concentrations of various variables.
  • The first graph shows the dilution to 30 mM, the second graph shows 60 mM, and the third graph shows 90 mM.
  • The concentrations of 2,2 bipyridine used are 0.0002316 M, 0.0004544 M, and 0.0006772 M from a stock concentration of 0.0099 M.


Adding Variables to 30:1 Lysozyme-AuNP Solutions

  1. Add 2.5 mL of each variable to each tube.
  2. Place 21 tubes in the oven at 36°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine.
  3. Place 21 tubes in the freezer at 4°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine.
  4. Place 21 tubes on the bench at 22°C: 30 mM, 60 mM, 90 mM of NaCl, KCl, MgCl2, CaCl2, MES, and citric acid, as well as 0.0006772 M, 0.0004544 M, and 0.0002316 M 2,2 bipyridine.


Synthesizing 30:1 Lysozyme-AuNP

  • The following figure describes how we made one tube of 30:1 lysozyme-AuNPs.
  • The sample was heated at 80°C for 4 hours.



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