User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/20

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TAR

  • Capture vector yeast recombinational cloning step initiated as per Lory protocol. Fresh cells were grown for 5hrs at 30C from a 1:100 ON innoculum in YPD.
  • LiAc chemically competent cells seemed to pick up the construct just fine (see positive and negative controls) with little uracil/cycloheximide reversion in the WT untransformed cells.
  • Plating indicates (after 12 hrs) very few visible colonies although there is evidence that they are beginning to form.


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