User:Jerome Bonnet/Notebook/Recombinases Notebook
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How to build a memory unit using recombination?
The major reason is that to my knowledge,it's the only recombination system to date where we can precisely control the switch from one state to another by controlling the expression or the activity of excisionase partner. It is highly sequence specific, structure of different integrases excisionases are availables, and a lot of biochemistry.
One attractive possibility is to use the Int/Xis system to switch DNA sequences like invertases, by positionning the Att sites into the same DNA molecule with an appropriate orientation (Pollock and Nash,j mol biol, 1983). This was also proposed by the Boston University/ Harvard IGEM 2004 team.
Invertases have been used by Ham et al. and are really promising tools. they just need to be improved.
what to do?