User:Jessica Karen Wong/Progress

T9002

Output Measurement Kit (for inverters, terminators, etc) Using GFP

<BBpart> T9002</BBpart>

• F2620 has Mfe/BB prefix tails
• Ligated into 3K3 and did correct transformation into DB3.1
• Waiting on seq verification
• E0240 has been PCR'ed with tails, looks the right size
• Digested E0240 S/N to ligate into F2620
• Will ligate into the correctly seq'ed F2620 colony

E0240

Promoter Tester with GFP

<BBpart> E0240</BBpart>

• Part is built
• PCRed with Mfe1 and Spe1 tails
• Successfully ligated into 3K3 - Sequence verifies that it's scarred 7/19
• Inserted a BB site between plasmid and RBS
• Made both E/S and E/X versions
• Has a 1bp mutation around 700
• Did mutagenesis and got a good number of colonies
• EX version has been confirmed correct - 8/9
• Made overnights to seq more ES colonies

I2057

Promoter tester with RFP

<BBpart> I2057</BBpart>

• Part is built (sequence verified)
• PCR-ed with Mfe1 and Nsi1
• Ligated in 3K3 (seq verified scarred) - 7/19 chose colony #4
• Inserted a BB site b/t plasmid and RBS
• Created both E/X and E/S versions
• E/S sealed w/ Not1 is seq verified - 8/6
• E/X sealed w/ Not1 is seq verified - 8/6 and 8/10

Ligated J116, J100, and R0040 into EX version from PCR

• R0040 in both EX and ES versions is perfect - 8/9

I2055

RBS tester with GFP

<BBpart> I2055</BBpart>

• Built and scarred - 7/31 and 8/2
• Inserted a BB site b/t Promoter and GFP
• Made both E/X and S/X forms
• Sealed both EX and SX with Not1 - waiting on seq
• Inserted B0034 and B0032 into both EX and SX versions of PCR (and hopefully prep), also B0030, B0031 into SX PCR
• I2055-SX-B0031 is seq verified 8/9

I2056

RBS tester with RFP

<BBpart> I2056</BBpart>

• Formerly called blue

• Built and scarred - 8/6, 8/10
• Inserted a BB site b/t promoter and RFP
• Created both E/X and S/X
• Sealed both SX (verified 8/14) and EX (8/14 and 8/15)
• Putting in B0034 and B0032 into both EX and SX versions