User:Jessica Perez/CloningWorkflow
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Planning
Before you start any cloning in the lab you first do the following:
- Decide what parts you need
- Design primers
- Draw up your expected plasmid. This can be done in Word or freely avalible DNA editor software such as <html>
<body> <a href="http://biologylabs.utah.edu/jorgensen/wayned/ape/"> APE</a> </body> </html>.
Source of DNA
<html> <canvas id="myCanvas" width="1000" height="1000" style="border:12px solid #000000;"> </canvas> <script>
var c=document.getElementById("myCanvas");
var ctx=c.getContext("2d");
ctx.fillStyle="#FFFFFF";
ctx.fillRect(400,10,200,75);
var c=document.getElementById("myCanvas"); var ctx=c.getContext("2d"); ctx.beginPath(); ctx.arc(200,200,100,0,2*Math.PI); ctx.stroke();
var c=document.getElementById("myCanvas"); var ctx=c.getContext("2d"); ctx.fillStyle="#FF0000"; ctx.fillRect(200,400,150,75);
ctx.font="20px Georgia"; ctx.fillText("Hello World!",10,50);
</script> </html>