User:Johnsy/Lipoprotein Modelling/Model Analysis
De Novo Synthesis Pathway and Degradation
Let us start by considering a simple system taking into account only cholesterol synthesis from HMG-CoA and it's degradation to either bile acids, steroid hormones, or other cholesterol derivatives. We can also model the action of statins as a competitive inhibitor of the enzyme HMG-CoA reductase, the main limiting enzyme of cholesterol biosynthesis. One of the key assumptions that is made is that the level of enzyme is constant (quasi-steady state approximation). Although this does not hold due to the genetic component, we will investigate the use of delay differential equations when considering a further extension to the model.
The equation we first consider is:
Solving for the fixed point of the equation is straightforward and we are left with the following steady state transfer function.
The parameters in the equation are shown below with their approximate values and references.
- V1 - The Vmax rate for HMG-CoA reductase, (Theivagt)
- Km1 - The michaelis-menten constant for HMG-CoA reductase, (Theivagt)
- Ki1 - Dissociation constant for average statin, (Flambers)
- dic - Degradation rate of cholesterol, estimated
- H0 - Average amount of HMG-CoA in the cell, assumed constant, (Corsini)
The graph in Figure 1 shows the effect of an increase in statin levels versus the steady state concentration of cholesterol.