User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/04/13

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Tuesday 13th April 2010

I made 1 lt of LB solid medium using:
Peptone 10g
Yeast extract 5g
NaCl 10g
Agar 15g

  • Procedure:
  • Weigh the ingredients but the agar and place them into a flask
  • Add 250ml of water to dissolve and shake until it looks even, meanwhile place 3.5g of agar in 4 schott bottles
  • Fill in a graduated cylinder with the mixture of the ingredients and water up to 1lt.
  • Add 250ml of the mixture with all the water to every schott bottle, then put the in the sterilizer to melt.



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