User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/04/15
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Thursday 15th April 2010
Making of the petri boxes by melting the schott bottles in the microwave for about 7 minutes at 30% potency, then cool them a little into water until it’s bearable to feel the with the check, now add ampicillin (100mg/ml) per ml now getting a concentration of 100ug/ml in the medium, and for kanamycin (30mg/ml) the same and the final concentration is 30ug/ml.
From the 2009 Kit Plate 1, well 10H. I extracted the Firefly luciferase - luciferase from Photinus pyralis Ba_I712019, for this I added 15ul of water to the well and I extracted all and placed it in a tube labeled: 2009 Ba_I712019 H10 Firefly.
PCR rtth (hot start):
........................1x (ul) ........4x (ul)
I made in total 4 tubes for the first reaction, 2 for the PCR and one negative control with water instead of DNA and the positive one with a 2kb DNA of something else.
1) Place the tubes with rx 1 in the termocycler for 4:30 minutes at 95ºC for denaturalization.
3) 5 mins at 72º so whatever is going on finishes the hold at 4º.