User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/06/29

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Tuesday 29th June 2010

I checked the plasmid extractions from the previous ligations on an agarose gel and came out as follows:

1º and 8º lanes are the DNA ladders
2º and 3º lanes are cI plasmid + GFP # 1
4º and 5º are cI plasmid + GFP # 2
6º and 7º are cI-PCR + GFP + Plasmid 18

There is plasmid on all colonies tested.

I still need to check the length of the plasmids inserts to be sure the ligations are the ones that I want.

For that I digested the plasmids with EcoRI and PstI as follows:
Reactive...1x [ul].....7x
DNA.........5.............-
BSA..........1.............7
Buffer2.....2............14
H2O.........10..........70
EcoR1......1.............7
PST1........1.............7
Total........20..........140



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