User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/07/29

iGEM Project name 1

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Thursday 29th July 2010 Today I digested the pBluescript II KS + plasmid and the cI inverter PCR with EcoRI-HF and PstI for posterior cloning of the inverter into the plasmid as follows: Reactive..............1x [ul] DNA....................20 BSA.......................1 Buffer2..................4 H2O....................11 EcoRI-HF...............2 PstI........................2 Total....................40 For the plasmid I did it twice. Then I checked the DNA quantities in an agarose gel for posterior ligation. 1º ladder 2º pBluescript II KS + plasmid 3º pBluescript II KS + cut with EcoRI-HF and PstI 4º pBluescript II KS + cut with EcoRI-HF and PstI 5º cI inverter PCR cut with EcoRI-HF and PstI