User:Jorge Arturo Zepeda/Notebook/iGem LCG-UNAM team 2010/2010/07/30

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Friday 30th July 2010

The plasmid digestions are not suitable for ligation, one is degrading the plasmid so I should throw it away but I’ll do it just in case, the other is partial. For both I raised the volume up to 50 ul and left it to cut all weekend!

Reactive.............1x [ul]
DNA..................---
BSA...................---
Buffer2..............+ 2
H2O..................+ 14
EcoRI-HF..........+ 2
PstI...................+ 2
Total..................50


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