User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/06/01

iGEM UNAM-Genomics-Mexico

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June 1st, 2010 1. PCR with RTTH PCR was done for Blue Promoter (2 reactions) and OGR as a control for primers. Reactives needed are as follows for one reaction with 2 ul of DNA each one: Reaction1 HPLC 12 Buffer 3.3x 6 Mg(Ac)2 3 dNTP’s 2 Primer FWD 2.5 Primer REV 2.5 DNA 2 Total 30 Reaction 2 HPLC 10.5 Buffer 3.3x 9 RTTH 0.5 Total 20 2. PCR product was purified using the High Pure PCR Product Purification Kit and an agarose gel 3% was run to verify PCR product. Lanes: 1,5) Ladder; 2) BP1; 3) BP2; 4) OGR