User:Jorge E. Buendia Buendia/Notebook/iGEM UNAM-Genomics-Mexico/2010/09/01
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September 1st, 2010
1. Run gel to verify purified PCR product of BBa_I20260 with the changed RBS and the double restriction (SpeI-XbaI) of this purified PCR product. This double restriction will not work for our purpose because it eliminates the J23101 promoter + RBS + GFP. I should’ve done the restriction only with XbaI.
Lanes: 1) Ladder; 2) Pure PCR product of BBa_I20260 with the changed RBS; 3) BBa_I20260 with the changed RBS double restriction (SpeI-XbaI); 4-8) Claudia's samples.