User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/09

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Plasmid extraction pBBRmcS5. From DH5α.

    • First lane control
    • Plasmids 7 lanes.

Image:9Mayo2011.pBBRmcS5.Extracción..JPG

Plasmid digestion

  • 7 digestions with HindIII
    • 2μL 10X Buffer 2 NE
    • 8μL H20
    • 10μL DNA Plasmid stock
    • 1μL Hind III.
  • Marks,
    • 1 tube without DNA
    • 1 tube without HindIII
    • 7 tubes with each plasmid stock.



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