- Extracting DNA from 2010 distribution.
- Plate 1. 7A. Plasmid pSB1T3 with 10μL H2O millinQ
- Transform DH5α competent cells.
- 1.-Cells defrosted on ice.
- 2.-100μL of cells for 3μL of DNA from plate.
- 3.-3 tubes were generated and transformed. 1 control with 50μL of cells.
- 4.-20 minutes on ice.
- 5.-Thermal shock at 42°C, 1 minute.
- 6.-5 minutes on ice.
- 7.-Add 1 ml of LB at each tube.
- 8.-1 hour at 37°C.
- 9.-Plate on Tet30-LB medium
- Odd cells stored at 4°C.