User:Jose Fabricio Lopez/Notebook/Logbook/2011/05/28

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  • Plasmid extraction again! of DH5α, this time trying to get better concentration.
    • Roche´s kit. Elution buffer: just 40μL.
  • Electrophoresis gel of:
    • PCR product with 80V, 90min, 2%.
    • Today's plasmid extraction. 80V, 90 min, 1%.

Picture not available.

Digestion

  • 4 double (XbaI & HindIII) digestions were performed, with Daniel´s extraction and mine extracted today.


1 2 3 4 5 Control
DNA 5† 5‡ 10† 10‡ 0
Enzyme HindIII (μL) 2 2 2 2 2
Enzyme XbaI (μL) 2 2 2 2 2
Buffer 2 10x (NEB) 2 2 2 2 2
BSA .2 .2 .2 .2 .2
H2O 8.8 8.8 3.8 3.8 13.8

†Daniel's extraction ‡Fabricio's extraction


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