User:Jose Fabricio Lopez/Notebook/Logbook/2011/07/08

iGEM 2011	Main project page Previous entry      Next entry
Objectives Plasmid restriction, with SpeI. Restriction I need a 2nd restriction for |pUX19-XkR5 plasmid, because the restriction of yesterday shows an unexpected pattern of 3 bands. I cut with SpeI because originally pUX19 has 2 sites, those sites flank insert of RepC-antisenseRNA, with that I expect 2 band: Larger band with 3.9kb Smaller band with 1.4KB(This is because insert for XKR5 plasmid has ~1.4Kb) Reactives Solutions SpeI 2μL Buffer 4 NEB 2μL BSA10x 2μL pUX19Extraction 5μL H2O 9μL Total 20μL Lane 1= Ruler Lane 2= pUX19XKR5 cut.