Objectives

• Grow in solid medium BBa_B0015 and liquid for extraction.
• See gel from extractions of pUX19 cut with SmaI.
• Digestion of pSB1AK3/BBa_B0015 with XbaI to see size.

Gel of SmaI restriction

I expect 6.2Kbp~

• Lane 1 Ruler
• Lane 2 Extraction 1
• Lane 3 Cut of extraction 1
• Lane 4 Extraction 2
• Lane 5 Extraction 3
• Lane 6 Cut of extraction 2
• Lane 7 Cut of extraction 3

Based on those results I will choose first extraction to PCR this match with the expected size too and it is a clean extraction.

Extraction

Extraction of plasmid with biobrick pSB1AK3/BBa_B0015, performed with miniprep.

I just need it to see concentration for digestion.

Digestion

Over night reaction.