User:Julia C. MacDougall/Notebook/Protein Modification/2015/02/06

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February 6th, 2015

Today, Andrew and I performed a heme extraction using the procedure from June 18th, 2014. Minimal changes were made; the changes that were made included

  • only making one solution instead of two in the initial setup
  • only performing the pH 2.5 extraction, not the pH 3.0 extraction.

After four extractions, the UV Vis still showed an absorbance peak ~400 nm. 1 mL To make the buffer, sodium dihydrogen phosphate (NaH2PO4) and potassium monohydrogen phosphate (K2HPO4) were used. The solution's final pH was roughly pH 2.3.


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