User:Karlena L. Brown/Notebook/PVOH Research/2013/02/08

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OBJECTIVES

  • Continue preparing polyvinyl alcohol microspheres for experimental analysis
  • Dilute Rhodamine 6G dye solution down to 1μM concentration for prepared microspheres
  • Prepare DSC samples for polyvinyl alcohol microspheres created

Microsphere PVOH 146K 110% CEC Laponite w/ DMHXLBR Information

Preparation of 110% Laponite Microspheres using PVOH MW 146,000-186,000 '
PVOH 146K added(g)0.9005
110% Laponite clay added (g)0.1002
Cellulose Acetate Butyrate added (g)0.6001
Glutaraldehyde added (mL)0.75
H2SO4 added (mL)0.25


Rhodamine 6G Dye Dilution Calculations

1μM Rhodamine 6G Dye Concentration

  M1V1 = M2V2
  1μM (RG6)x 25mL = (92μM)V2    V2 = 271.74μL

DSC Analysis: Exchanged Clays & PVOH 146K Microspheres

DSC preparation and protocal involving instrumentation and usage can be found on 9/17/12 entry.

Sample Name Sample Mass (mg) Pan / Lid Mass (mg)
NaMT3.1648.13
50% CEC NaMT w/ Bu3HdP+2.9048.91
110% CEC NaMT w/ DMHXLBR3.0150.14
Laponite3.0650.18
110% CEC Laponite w/ DMHXLBR3.0849.52
90:10 PVOH 146K Laponite Dust2.8948.87
90:10 PVOH 146K Laponite Medium Clumps2.9648.89
90:10 PVOH 146K Laponite Large Clumps3.0748.18

Notes

  • After preparing the microspheres from the PVOH 146K Laponite, many of the spheres were not spherical. Large clumps of all sizes were produced rather than microspheres.
  • The microspheres prepared from PVOH 146K Laponite were rigid white crystalline structures with stacked layers.
  • Due to large sizes of the microspheres, they had to be ground up using a mortar and pestle before adding the 1μM rhodamine 6G dye solution.
  • Also DSC was taken of the different sizes of the microspheres to determine if the structure, function, and capability of the microspheres had changed due to extensive clumping.
  • When going through the process of preparing new microspheres from PVOH 146K 110% CEC Laponite w/ DMHXLBR, due to slowed vacuum filtration the sample was placed in a vial with the last solvent used for cleaning, methanol.
  • When freezing the new microsphere sample in liquid nitrogen, PVOH 146K 110% CEC Laponite w/ DMHXLBR, it was frozen in a medium of methanol and vacuum filtrated to remove the methanol residue.



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