User:Karmella Haynes/Notebook/ASU courses/2012/01/24

BME194 Syn Bio Demo

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1/24/12 Transformations: streaks Cultures: O2-GFP, O1-GFP, Osym-GFP, LacI-O2 for minipreps (5 mL); Banana for generator test (5 mL) Transformations > Transformations from 1/23 worked! > Streak single colonies onto agar, grow all day. Cultures > Minipreps, 5 mL ea. O2-GFP (Amp) O1-GFP (Amp) Osym-GFP (Amp) LacI-O2 (Kan) > Banana generator test (from streaks): Banana Banana + 5μL isoamyl alcohol RFP1 RFP1 + 5μL isoamyl alcohol