User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/19

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12/19/09 ✓ Minipreps: KAH117(3), 118(4), 119(1) Minipreps > Check with E/P digests Reagent Volume Expected: 1-3. KAH117 = 7758 4-7. KAH118 = 7930 8. KAH119 = 8002 15 μL/lane; 1% agarose DNA(plasmid) 2.0 μL 10X buffer 1.5 EcoRI 1.0 PstI 1.0 dH2O 9.5   15 μL --> 37°C/ ~15 min.