User:Karmella Haynes/Notebook/BioBrick cloning/2009/12/28

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12/28/09 ✓ Cultures: KAH136(2), 137(4), 138(4); retransformations KAH93(2), JDS42(2), JDS43(2) ✓ Oligo annealing: BglII adapters for RFP-rep inserts into JDS42 & 43 Oligo annealing Ad BglII-EcoRI Ad PstI-BglII DNA (oligos, 100 μM) up to 18 μL (3 μL ea.) 10x annealing buffer 2.0 dH2O ---   20 μL --> 100°C (water bath)/ 5 min.; Cool to R.T. overnight