User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/02

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1/02/10

  • ✓ Minipreps: KAH117 (4)

Minipreps
> Check with E/P digests

Reagent Volume Expected:
1-4. KAH117 re-picks = 7758
E/P digests 1/02/10
15 μL/lane; 1% agarose
DNA(plasmid) 5.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 6.5
  15 μL --> 37°C/ ~15 min.



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