User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/21

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01/21/10

  • ✓ Cultures: KAH139, 140, 141/V0201 (2 each)
  • ✓ Miniprep digests: KAH94, 95/ JDS48, 49; BglII

Miniprep Digests
> Check KAH94, 95/ JDS48, 49 with BglII

Reagent Volume Expected:
1-6. KAH94/JDS49 = 7859, 4022, 346
7-12. KAH95/JDS49 = 7859, 4094, 346
13-18. KAH94/JDS48 = 7849, 4022, 346
19-24. KAH95/JDS48 = 7849, 4094, 346
25. JDS48 = 7509
26. JDS49 = 7519
27. KAH94 = 4022, 3540, 346
27. KAH95 = 4094, 3540, 346
Hover name
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
BglII 0.5
dH2O 11.0
  15 μL

--> 37°C/ ~15 min.
--> None are correct. There could be recombination deleting the HS4 insulators.



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