User:Karmella Haynes/Notebook/BioBrick cloning/2010/01/23

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01/23/10

  • ✓ Minipreps: KAH139, 140, 141/ V0201
  • ✓ Cultures: KAH140, 141/V0201 (4 each)

Minipreps
> Check with XbaI digests: expected size insert = 660 bp + insert; plasmid remainder = ~4500

Reagent Volume Expected:
Vector = 4500
1,2. KAH139/V0201 = 3655
3,4. KAH140/V0201 = 3599
3,4. KAH141/V0201 = 3187
XbaI digest 1/23/10
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.

--> Pick more KAH140, 141/V0201 colonies (4 each)



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