User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/20

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03/20/10

  • ✓ Minipreps: KAH142/V0201 (8)

Minipreps
> Check with XbaI digest
> XbaI fragment (insert) will have an extra 641 bp from vector sequence; remaining vector = 4516

Reagent Volume Expected:
1-8. KAH142/V0201 = 4571 (5212)
XbaI digest 3/19/10
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
dH2O 10.5
  15 μL --> 37°C/ ~15 min.

--> None look correct. Re-do assembly (new KAH142 N/S digest)


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