User:Karmella Haynes/Notebook/BioBrick cloning/2010/03/22

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03/22/10 ✓ Cultures: KAH142/V0201 (4) ✓ Assembly: KAH143 Assemblies KAH143: DT03/(E/S)/1813 ✓ + KAH010/(E/X)/~3234 ✓ --> DT03 = 27.7 ng/μL --> KAH010 = 25.1 ng/μL > Ligations Ligation Plate results (lig : neg crtl) 03/23/10 1. DT03/(E/S)/1813, 28 ng + KAH010/(E/X)/~3234, 25 ng KAH143 10:1 (Pick 4) 2. KAH010(E/X)/ 25 ng ~10 colonies   1 2 Insert DNA 1.0 --- Vector DNA 1.0 1.0 2x lgn buf (Roche) 5.0 5.0 T4 ligase (NEB) 1.0 1.0 dH2O 2.0 3.0   10 μL 10 μL --> R.T./ ~10 min., Add 50 μL Z-DH5α