User:Karmella Haynes/Notebook/BioBrick cloning/2010/07/12

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07/12/10

  • Oligo annealing: BioBrick flyNLS



Oligo annealing

  1. flyNLS: flyNLS 1, flyNLS 2


DNA (oligos, 100 μM) 4.0 (2.0 each)
10x annealing buffer 2.0
dH2O 14.0
  20 μL --> 100°C (water bath)/ 5 min.; Cool to R.T. overnight



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