User:Karmella Haynes/Notebook/BioBrick cloning/2010/07/26

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07/26/10

  • ✓ Sequencing order: fly mCherry mutagenesis clones



Sequencing order (Genewiz standard)
--> Forward primer = M13F(-21) (Genewiz)
--> Reverse primer = M13R (Genewiz)

  1. flymCh-01 F: good
  2. flymCh-01 R: good
  3. flymCh-02 F: not checked
  4. flymCh-02 R: not checked
  5. flymCh-03 F: not checked
  6. flymCh-03 R: not checked
  7. flymCh-04 F: not checked
  8. flymCh-04 R: not checked

--> Keep clone #1
--> Next, add BioBrick ends via PCR and clone into V0120



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