User:Karmella Haynes/Notebook/BioBrick cloning/2010/08/09

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08/09/10

  • ✓ Minipreps: KAH160-170/KAH015 (1 each)
  • ✓ Cultures: KAH160-170/KAH015 25 mL cultures for midipreps



Minipreps
> Check with KpnI digests

Reagent Volume Expected:
Backbone = 7489
Empty vector frag. = 913
1. KAH160/KAH015 = 2060
2. KAH161/KAH015 = 2060
3. KAH162/KAH015 = 2060
4. KAH163*/KAH015 = 710, 2319
*Has a KpnI site
5. KAH164/KAH015 = 3047
6. KAH165/KAH015 = 1904
7. KAH166/KAH015 = 1904
8. KAH167/KAH015 = 1904
9. KAH168*/KAH015 = 710, 2163
*Has a KpnI site
10. KAH169/KAH015 = 2871
11. KAH170/KAH015 = 1868
8/09/10 mp digest
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
KpnI 1.0
dH2O 10.5
  15 μL --> 37°C/ ~15 min.
 
 
 
 




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