User:Karmella Haynes/Notebook/BioBrick cloning/2011/07/11

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07/11/11

  • ✓ Miniprep digests: KAH210, 211, 212



Minipreps
> Check with E/P digests

Reagent Volume Expected:
1,2. KAH210 = 2398
3,4. KAH211 = 1321
5,6. KAH212 = 1621
Miniprep digests 7/11/11
15 μL/lane; 1% agarose
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.



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