User:Karmella Haynes/Notebook/BioBrick cloning/2015/08/26

Karmella's BioBrick Cloning

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08/26/15 Minipreps - CRISPR library Cultures - CRISPR library (round 2) Minipreps - CRISPR library Minipreps Sigma GenElute kit, elute w/ 75 μL elution sln. L34-032 L34-040 L34-094 L34-096 Measure conc.'s Sample OD260 260/280 ng/μL 1. L34-032 0.167 1.863 167.3 2. L34-040 0.026 1.908 26.4 3. L34-094 0.032 1.901 32.4 4. L34-096 0.142 1.859 142.4 Sequencing: Order 10437 L34-032, P163 L34-040 " L34-094 " L34-096 " Cultures - CRISPR library (round 2) Re-grow cultures for clones with failed sequencing runs From DNASU Order 10333 Note: DNASU sampling from the 96-well block is top to bottom, then left to right A1 = G34-001, A2 = G34-002, A3 = G34-003, etc. Low phred scores (<700) generally corresponded with the lowqual reads observed in Benchling Scrape bacteria from the original spot agar plates; grow as 5 mL culture for miniprep (overnight) Clone, well (agar spot position), phred score G34-024, H3, 746 G34-067, C9, 444 G34-079, G10, 888 RESULTS(08/27/15) All three grew