User:Karmella Haynes/Notebook/PcTF Genomics/2012/10/23

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10/23/12

  • Thaw new cell stocks: K-562 and SK-N-SH from ATCC


New Cell Stocks

  • K-562: recommended media is IMDM (10% FBS, 1% pen/strep)
  • SK-N-SH: recommended media is EMEM (10% FBS, 1% pen/strep)
  • Thaw and spin down in 10 mL of the appropriate complete medium (3 min, 1000 rpm, room temp)
  • Resuspend pellet grow in 4 mL and grow in a 25 cm small starter flask for 2 days (5% CO2, 37°C)




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