User:Karmella Haynes/Notebook/PcTF Genomics/2012/10/25

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10/25/12

  • Expand cell cultures: K-562, SK-N-SH



Expand cultures

  • Checked cells under scope. Good amount of cell division
  • K-562 (suspension): transfer the 4mL culture to 6 mL IMDM (10% FBS, 1% pen/strep) in a 75 cm flask
  • SK-N-SH: aspirate old medium, wash with 2 mL 1xPBS; detach with 0.5 mL Trypsin media (3 min.); collect in 3 mL EMEM (10% FBS, 1% pen/strep), add to 7 mL medium in 75 cm flask




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