User:Karmella Haynes/Notebook/PcTF Genomics/2014/05/19

From OpenWetWare

Jump to: navigation, search
Pc-TF Genomics Main project page
Previous entry      Next entry


05/19/14

  • Cell culture: thaw KAH126 and KAH154



Thaw KAH126 and KAH154

  • Pellet 1mL thawed cells in 5 mL FBS
  • Discard supernatant, leave 100 uL, disrupt the pellet
  • Resuspend in 4 mL 20% FBS/ 1% P/S
  • Grow in T-25 flasks



Personal tools