User:Karmella Haynes/Notebook/Polycomb project/2010/02/06

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02/06/10

  • ✓ Polycomb-ATF lines: passage, expansion, Dox induction



Polycomb ATF lines
> For each cell line (8 total)
--> Harvest ~1x106 (from 6-well plates) in 2 mL medium
--> Expansion: Plate 1 mL in a 1x 10 cm plate (grow up for frozen stocks)
--> Passage: Plate 0.5 mL in 2 wells of a 6-well plate (for Dox induction and expression analysis)
--> After cells attach, check for background RFP expression (some showed leaky expression in very few cells)
--> Add 1 μg/mL Dox to each sample



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