User:Karmella Haynes/Notebook/Polycomb project/2010/02/17

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02/17/10

  • ✓ Polycomb-ATF RT-PCR cultures: split plates
  • ✓ Pc-ATF lines: split confluent plates (KAH128-8, 132-8) 1:10 in 10 cm dishes
  • ✓ Protein prep: Pc-ATF lines (+Dox, 2 days)



RNA prep/ RT-PCR cultures
> Dox induction so far: 2 days
> Split plates: split plates by 1/2 (double number of plates)
> Add dox (final = 1 μg/mL) to Dox+ samples



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