User:Karmella Haynes/Notebook/Polycomb project/2010/05/21

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05/21/10

  • ✓ ChIP: chromatin prep & sonication for KAH126-1 and U2OS samples
  • ✓ Western blot: continued from 5/18/10
  • ✓ Cell culture: freeze down KAH132 lines (3) (growth assay lines)
  • ✓ Protein preps: Pc-ATF neg ctrl lines
  • ✓ Growth assay: do cell count (day 4)



Growth assay (day 4)
> Do cell counts for the following:

  1. KAH126-1
  2. KAH126-3
  3. KAH126-4
  4. KAH132-8
  5. FTRx
  6. KAH126-1 +dox
  7. KAH126-3 +dox
  8. KAH126-4 +dox
  9. KAH132-8 +dox
  10. FTRx +dox

--> Checked cells for RFP expression. KAH126-4 shows no RFP.
> Trypsinize cells with 200 μL Trypsin buffer
> Resuspend cells with 800 μL medium (1 mL total volume)
> Dilute samples 1/4 for Scepter counting; add 25 μL cells to 75 μL PBS

> Refresh medium and dox in other wells


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