User:Karmella Haynes/Notebook/Polycomb project/2010/09/09

From OpenWetWare
Jump to navigationJump to search
Project name Main project page
Previous entry      Next entry

09/09/10

  • ✓ p16 time course: Induce 4-day samples with 1 μg/mL dox (3 pm)
  • ✓ Order oligos: new RT-PCR primers for HHEX
  • ✓ Plasmid cultures: pick three colonies each from KAH160/015 and KAH170/015 plates (8/07/10)


Pc-ATF expression in other cell lines
> Casey can get expression from KAH165/015 but not from KAH160/015 and KAH170/015 in HepG2 cells
--> Mutation in the viral backbone?
> Pick three colonies each from original cloning plates
> Tomorrow: order sequencing using Genewiz primers:

  1. CMV-forward: reads into HIV-1 5' LTR (upstream)
  2. BGHR: reads into HIV-1 5' LTR (downstream)



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Karmella_Haynes/Notebook/Polycomb_project/2010/09/09&oldid=1006299"