User:Karmella Haynes/Notebook/Polycomb project/2010/12/17

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12/17/10

  • ✓ ChIP: 128-8.3, 129-4 chromatin preps (flash freeze and do ChIP when myc beads arrive)
  • ✓ ChIP: IP's; was and elute; DNA purification day 1



ChIP: wash & elute IP's
> Washes: Follow Casey's (modified) protocol for all samples (see 12/01/10)
> Elution & DNA purification: Follow Qingqing's protocol (see 12/01/10)
> Samples (all form. x-linked chromatin)
1/2. 126-1: + 30 μL αmyc-beads (3400); + 30 μL mouse IgG-beads (3420)
3/4. 130-4: "
5/6. 132-8: "
7/8. FTRx: + 5 μL αH3K27me3 (07-449); + 5 μL rabbit IgG (sc-2027)



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