User:Katherine Running/Notebook/Biology 210 at AU

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Katherine Running's Laboratory Notebook- Bio 210

Invertebrates 2/11

Worm Examples/Types

Earthworm-segments tighten and extend. Slinky kind of motion, worm lengthens and then pulls end of body towards the front of the body, slowly moving forward.
Flatworm-slow, creepy, flexible. glide, cautious, look around with their head multiple times before deciding to move, like checking the road before you cross, move in direction of head.
Nematode-fast, whip-like motion, angry serpent looking thing.

Five Jar Organisms

1.Bee- Arthropoda Insectca
2. Centipede- Arthropoda Chilopoda
3. Crab-Arthropoda Malacostraca
4.Tarantula-Arthropoda Arachnida
5. Millipede- Arthropoda Diplopoda

Transect Five Invertebrates

Organism Length in mm Number in sample Description
Top Niche-Arthropoda Insecta Hymenoptera 2.5 millimeters 1
Top Niche-Arthropoda Insecta Dermaptera 5 millimeters 1 long antennae, 6 legs, segmented, 2 tails, long and skinny
Bottom Niche-Arthropoda Diplopoda 15 millimeters 3
Bottom Niche-Arthropoda Insecta Homoptera 10 millimeters 1

Plantae and Fungi 2/4

Transect Sample Plants Location and # in Transect Description (size and shape) Vascularization Specialized Structures Mechanisms of Reproduction
#1 Clover Angiosperm Edge of flowerbed and grass Simple root system. don't see seeds. flower. dicot. picture's will be uploaded later. vascularized None flowers
#2 Rose Angiosperm Bush number 1, 8 bushes thorny bush with flower bulbs, net-like leaves, dicot vascularized sexual reproduction organs bulbs
#3 Grass angiosperm Grass flower, parallel leaves, crazy root system, monocot vascularized none flowers, seeds, sexual
#4 flowerbed thicker, waxy, red stems, crazy root system, probably flowers, monocot vascularized None sexual reproduction
#5fern flowerbed, fern parallel leaves, long, spindly, narrow vascularized None spores, sexual

Microbiology and Identifying Bacteria with DNA Sequences-1/28/15

Quantifying and Observing Microorganisms

Dilution Agar Type # of Colonies Conversion Colonies/mL
x10^-3 Nutrient 1052 x10^3 1,052,000
x10^-5 Nutrient 2231 x10^5 223,100,000
x10^-7 Nutrient 716 x10^7 7,160,000,000
x10^-9 Nutrient 425 x10^9 425,000,000,000
x10^-3 TET 241 x10^3 241,000
x10^-5 TET 48 x10^5 4,800,000
x10^-7 TET 12 x10^7 120,000,000
x10^-9 TET 38 x10^9 38,000,000,000

Less colonies grew on the tet-plates, but they were bigger. Maybe because they had less colonies to complete with for nutrients? We had no fungi grow. Only bacteria. There were two visible types of bacterium, one we called yellow, and one we called white. However, under the microscope the white one was actually two different bacterium coexisting. Both white and yellow bacterium grew on the TET plates, but more yellow ones were able to survive than white ones.

No Archaea are expected to survive on the agar plates. Archaea live in extreme conditions.

Colony Label Plate Type Colony Description Cell Description Gram +/-
NY Nutrient punctiform, flat, small diameter +
NW Nutrient punction, flat, larger, squiggly edges -
TY TET circular, umbinate +&-
TW TET circular, convex +*-

Hay Infusion Culture Observations

Gritty, slimy, watery, smelly, stronger manure smell, bugs flying around, water level lower. Likely things are decomposing.
Hay Infusion-Transect 5, week 2, picture 1

Hay Infusion-Transect 5, week 2, picture 2

Hay Infusion-Transect 5, week 2, picture 3

Identifying Algae and Protists-1/21/15

Hay Infusion Culture Observations

Soil settled to bottom of jar. Some leaves and grass still floating. Smells like manure. Film on top of the water.

Good observations of the hay infusion and awesome documentation for the niche 1 protists. I would have liked to see photo documentation for the second niche protists as well. Very complete lab notebook so far... great job! Make sure you date every entry so that as the notebook gets longer, things don't get confused as to which week they are from.
Hay Infusion-Transect 5, week 1, picture 1

Hay Infusion-Transect 5, week 1, picture 2

Hay Infusion-Transect 5, week 1, picture 3

Hay Infusion-Transect 5, week 1, picture 4

Niche 1-Top of Hay Infusion

Organism 1- Colpidium or Paramecium Bursaria

organism zooms around very quickly. Around 37 micrometers in length. stretches and reaches to eat food. clear. No flagella visible, so likely has cilia. Body oblong/oval shape.

Organism 2-Gonium

Many celled colony. Green. flat. Many gonium clumped together. 500 micrometers in length.No image.

Organism 3-Unidentified

110 micrometers in length. colorless. large mouth that opens and closes, as if gulping water. only swims in direction of skinny end/end with mouth. slower than Organism 1.

Niche 2-Bottom of Hay Infusion

Organism 4-Spirostomum

worm like. elongated body/long and narrow. No apparent flagella, though may not have cilia. wiggles. 25 micrometers in length. no image.

Organism 5-Unidentified

round head about 2.5 micrometers in length. 6-7 micrometer long tail. swims in direction of head. head is dark blue/black colored. tail is also dark. tadpole shaped. or balloon on string. not seen eating or interacting with other organisms. No image.

Biological Life at AU-1/14/15

Thanks for including the volvocine line information in the notebook too! ML

Part 1: Green Algae Evolution

Characteristics Chlamydomonas Gonium Volvox
Number of Cells in Colony 25 32 too many
Colony Size (μ‏m) 2.5/cell, 25/colony 50 180
Specialization of Cells None A little bit of specialization Specialized
Mechanism of Motility extremely fast! flagella don't move don't move
Isogamous or Oogamous Isogamous Isogamous Oogamous

Part 2: Observing Our Transect

Great job on your transect documentation and aerial layout. ML

Data and Observations:

Biotic: clover, grass, bush type 1, bush type 2, fern
Abiotic: woodchips, soil, snow, rocks, leaves

Bush Type 1

Bush Type 2




Corner A looking at Corner B

Corner A looking at Corner D

Corner D looking at Corner A

Corner C looking at Corner D

My First Post

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