User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/08

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Contents

Objective

Continue with ligation attempts

Bench work

  1. User:Daniel Catt ran an analytical gel of ligation reactions
  2. Re-plate electroporated DH10B

Results

Transformations

  • 3 colonies on 400μL plate of NovaBlue transformed with V(His)+I
  • 3 colonies on 100μL plate of electroporated DH10B with V(His)+I
    • 1 colony on negative control (V(His) electroporated into DH10B)

Gel

no bands visible for ligation reactions

Image: DNA gel 070811labeled.jpg


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