User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/07/13

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Objective

Start making competent cells of expression strain, ER2566. We're using a new protocol containing hexamminecobalt chloride in the transformation buffer and adding DTT in the process. See Hanahan, D. (1983) JMB 166 557-580.

Bench work

  1. Glycerol stocks (done by Daniel Catt)
    • Prepared glycerol stocks with 3mL cultures from yesterday
      • #88 pTXB1+BSA Colony 1
      • #89 pTXB1+BSA Colony 2
      • #90 pTXB1+BSA Colony 3
      • #91 pTXB1.His+BSA Colony 1
      • #92 pTXB1.His+BSA Colony 2
      • #93 pTXB1.His+BSA Colony 3
  2. Competent cell prep (done by Daniel Catt)
    1. innoculate 250 mL SOB with 6 colonies from E. coli ER2566 plate from a few weeks ago
    • → 18°C w/ shaking
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