User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/01

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AU CHEM-570 Lab Prep Main project page
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Objective

  • Determine whether Ins_His_after_CBD and Rins_His_after_CBD primers will appropriately match sequence around C-term of CBD in pMXB10 plasmid
  • If acceptable, QuikChange of pMXB10 plasmid with above His-tag primers
  • Transform test ligations from 7/28


Bench work

  1. Verified complementarity of His-tag primers
  2. QuikChange protocol with pMXB10
  3. Transformation of:
    • New ligation of pTXB1 (digest by EcoRI, BamHI, arctic phosphatase) 7/28
    • New ligation of pTXB1 (digest by EcoRI, BamHI) 7/28
    • pTXB1 (digest by EcoRI, BamHI, arctic phosphatase) 7/26
    • pTXB1 (digest by EcoRI, BamHI) 7/26

Results

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