User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/08/12

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Bench work

  1. Ran DNA gel (1% agarose) with ligation samples from yesterday
    • Lane 1: Ladder (10μL)
    • Lanes 2 & 3 skip
    • Lane 4: 5μL pKK223-3 BamHI digest, ligation (with 10μL in ligation reaction)
    • Lanes 5 & 6 skip
    • Lane 7: 5μL pKK223-3 BamHI digest, ligation (with 15μL in ligation reaction)
    • Lanes 8 & 9 skip
    • Lane 10: 5μL pTXB1 test PCR from 8/10 (with Pfu Turbo buffer 'A')
    • Lanes 11 & 12 skip
    • Lane 13: 5μL pTXB1 test PCR from 8/10 (with Pfu Turbo buffer 'B')

Results

Gel

Image: DNAgel_8.12.2011_labeled.jpg

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