User:Khyra A. Neal/Notebook/Chem 571/2014/10/07

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October 7, 2014 Tasklist 1. Solution Preparation 42 μM = 0.6 g/L Lysozyme add 0.015 g Lysozyme to 25 mL deionized water 30:1 lysozyme-gold colloid Add 0.5 mL 250 mM HAuCl4 , 1 mL 42 μM (0.6 g/L) Lysozyme, and 3.5 mL HPLC-grade H2O to 5 culture tubes Place in oven for 4 hours 50 mm NaCl 50 mM Tris buffer solution was made by adding: 0.3030g of Tris buffer 0.1461g of NaCl Add to 50 mL distilled water 2. Bradford Analysis Solutions were prepared on October 1, 2014 20 μL sample solution 200 μL Bradford Reagent (diluted 1:3 with Tris buffer) 780 μL 50 mM Tris/ 50 mM NaCl Buffer Solutions were vortexed in a 1.5 mL centrifuge tube and equilibrated for 5 minutes Ran UV-Vis between 400-800 nm using PS cuvettes An Undialyzed Lysozyme Bradford analysis was also ran. Transfer remaining dialyzed solutions to an extraction vial. 3. pH Measurements of H+ containing solutions pH measurements Sample pH 0.25 mM HCl (4) 8.062 Lysozyme (4) 8.134 4. Ca2+ ISE of Ca2+ containing solutions Substance Potential (mV) Colloid Sample 1 73.5 50 mM CaCl2 (1) 74.9 Colloid Sample 2 51.2 500 μM CaCl2 91.4