User:Khyra A. Neal/Notebook/Experimental Biological Chemistry II Notebook/2015/03/04

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March 4, 2015

Objective

  • Analyze Reaction Products using TLC and NMR

Description

  1. TLC
    • The completion of the reaction was tested by spotting a TLC and using hexane and chloroform (4:1) as the solvent. Based on TLC, it can be determined that some product formed according to the separation on the plate compared to the starting material.
  2. NMR
    1. The reaction was removed from heat and was stirred continuously for 2 hours after excess water was added. The reaction mixture was filtered and the solid obtained was dissolved in 5 mL ethyl acetate to recover the nanoparticles.
    2. About 10 drops of the "benzophenone" sample was evaporated on a watch glass, redissolved in Chloroform-D, then transferred to an NMR tube for analysis.

Data

Image:Benzophenone_3-4.jpg

The NMR spectrum shows that more product formed with the citrate gold nanoparticles than with the protein-supported gold nanoparticles.


Notes

  • Double hydrogen peroxide is going to be added in the next reaction.


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