User:Klare Lazor/Notebook/Chem-496-001/2011/11/01

From OpenWetWare

Jump to: navigation, search
Biomaterials Design Lab Main project page
Previous entry      Next entry


- protein not going to be ready to explore as much as we would like

- start to work out some of the kicks that we are seeing with nanoparticle formation

-been getting a gray matter figure our what is going on

- main focus on gray matter what problem we are seeing. what can we do. im going to try making.

- will still do some intein chemistry and things like that


Today: Another PCR, gold reaction (note how you prepared samples protein and water) and (1mL protein 8ml water 1ml 3molare hcl) next week expression.

  • we add acid how regular protein aggregates form and can determine whether gold affecting or acidity.
  • Some one make a gold stock and some one make a bsa stock


1st time out of oven -nothing happened to either 2nd -after second time in oven for 50 minutes black clumps appeared in test tube 1 and 3 had black - 2 and 4 didnt have anything 3rd time out oven -test tube 1 and 3 contained material. seeped to botoom -2 and 4 had nothing

Test tube solution composition

  • 1mL(2.9mM gold) and 1mL (15microMolar BSA old) and 8mL of H20
  • 1mL(2.9mM gold) and 1mL (15microMolar BSA old) and 1mL of HCl 3M + 7mL of H20
  • 1mL(2.9mM gold) and 1mL (15.5microMolar BSA new) and 8mL of H20
  • 1mL(2.9mM gold) and 1mL (15.5microMolar BSA new) and 1mL of HCl 3M + 7mL of H20


  • Add data and results here...


This area is for any observations or conclusions that you would like to note.

Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.

Personal tools