User:Klare Lazor/Notebook/Chem-496-001/2012/02/01

From OpenWetWare

Jump to: navigation, search
Biomaterials Design Lab Main project page
Previous entry      Next entry


Contents

Objective

  • synthesize AUNP at a concentration of 60 HAUCl4 to 1 BSA
  • prepare stock solutions

Description

  • Stock Solutions
    • HAuCl4 in 50mL solution
      • 2.7mM= 0.0027mols/L x 0.05 L = 1.35E-4 mols HAuCl4
      • 1.35E-4 mols HAuCl4 x 337.79 mols = 0.0458789 grams of HAuCl4
    • BSA in 50mL solutions
      • 1.5µM=0.000015mols/L x 0.05L = 7.5 E-7 mols
      • 7.5 E-7 mols x 66,000 g/mol = 0.0495 grams of BSA


  • BSA/AuNP Preparation of 60:1 based off stocks above
    • In oven for four hours


Calculations for dilutions of 60:1 in 10 mL

  • (0.906ml HAuCl4)(0.0027mols/L)= (10mL)(x)
    • x=0.000245mols/L of HAuCl4 in 10mL of water total volume
  • (60)/(1)=(0.000245)/(x)
    • x=0.000004 mols/L of BSA
  • (x)(.000015mols/L)=(10mL)/(0.000004 mols/L)
    • x= 2.666 mL of BSA in 10mL of water total volume


  • So,
    • 2666 µL of HAuCl4
    • 906 µL of BSA
    • 6428 µL of H20

Check on ratio of concentration= (0.000009mols/L)/ (0.00000015mols/LBSA) = 60/1

Data

  • nothing, bsa stock was to dilute to produce any nano particles

Notes

  • No nano particles were made due to lack of HAuCl4.
Personal tools